Nutrition and food tests (CCEA)

Part of Biology (Single Science)Living processes

What are biological molecules?

The food we eat – our diet – is made up of different biological molecules which give us energy and contain chemicals we need to grow and repair and help cells function in our body.

Carbohydrates

Carbohydrates provide energy. There are two types - simple and complex.

Simple carbohydrates are sugars, like glucose and lactose, and are found in food like biscuits or energy bars.

Complex carbohydrates are:

Cellulose – found in plant cell walls providing support to the cell

Starch – an energy store in plants. Broken down by amylase into glucose, for respiration

Glycogen – an energy store in animals. Can be broken down into glucose when needed

Complex carbohydrates can be found in foods including whole-grain products, such as bread, rice and pasta, some fruits, legumes, and starchy vegetables, such as sweet potatoes.

Fats

Fats are an energy store, providing twice as much energy as carbohydrates and proteins.

They are made up of fatty acids and glycerol.

Foods high in fat include cheese, butter and oils.

Back to top

What are proteins made up of?

Proteins are made up of long chains of amino acids and are essential for growth and repair of cells.

They serve as:

  • structural molecules eg in muscles, skin, nails and

  • functional molecules eg enzymes, hormones, antibodies

Foods high in protein include fish and eggs.

Back to top

Reagents and food testing

Different tests can detect carbohydrates, proteins, and lipids in food.

These tests use a chemical (reagent) that changes colour if certain molecules are present.

Sometimes, the food needs to be crushed or mixed with water before adding the reagent.

Testing for protein/amino acid
Figure caption,
Testing for protein/amino acid
Food sampleReagentMethodInitial colourColour of positive result
Reducing sugarBenedict'sAdd Benedict’s solution to the food and heat in a water bathBlueBrick red precipitate
StarchIodine solutionAdd iodine solutionYellow-brownBlue-black
Protein/amino acidsBiuretAdd Biuret reagentBlueLilac/purple
FatEthanolAdd ethanol to the food, then add waterColourlessWhite emulsion
Back to top

Practical - How to investigate the energy content of food

A simple investigation can be conducted to investigate the energy content of a food sample.

Procedure

  1. Add 20cm³ of water to a boiling tube in a retort stand.
  2. Record the starting temperature.
  3. Ignite 1g of food on a mounted needle.
  4. Hold the burning food under the boiling tube until fully burned (relight if needed).
  5. Record the final water temperature.
  6. Record results in a table.
  7. Repeat steps 1-6 with different foods.

Results:

FoodAt start (°C)At endDifference
Corn crisps204222
Potato crisps203616

Analysis: Use the formula to calculate energy released. This formula will be provided in the exam.

1cm³ of water = 1g

Energy (J) = mass of water (g) x temp rise (°C) x 4.2.

Corn crisps = 20g x 22 x 4.2 = 1848J

= 1.8kJ

How much energy was in the potato crisps? Answer = 20g x 16 x 4.2 = 1344J

= 1.3kJ

Conclusion:

The corn crisps had more energy because they had a larger increase in temperature.

Sources of error:

The results obtained from this experiment are usually lower than the actual energy content of the food because during the experiment, the entire food sample may not be burned, some energy is lost to the air and some is used to heat the glass of the boiling tube.

Improvements:

  • reduce heat loss to surroundings by using a
  • use a
  • stir water: stirring improves accuracy because it ensures even heat distribution by preventing temperature variations within the water. Without stirring, hot and cold spots can form, leading to inconsistent readings depending on where the thermometer is placed. By stirring, the heat is evenly distributed, ensuring that the temperature measurement is more reliable and representative of the whole test-tube.

Independent variable: type of food

Dependent variable: temperature / °C

Controlled variables: mass of food, mass of water, distance of burning food from water

Back to top

Test your knowledge

Back to top

Watch: Biological Molecules

Back to top

More on Living processes

Find out more by working through a topic